Direct Analytical Sample Quality Assessment (DASQ) for...

There are several important factors to increase the chances that a proteomic study based on CSF biomarkers becomes successful. For proteomics analysis, the standardisation of CSF collection protocols is a mandatory step to search and identify new biomarkers indicators of a disease status. This point represents a milestone especially when samples are collected and stored in different centres. There is the need to select and include, in biomarker research studies, only well characterized and high quality samples with a documented sample collection and handling. Our study undelines the importance of a standardized protocol, based on some specific procedure of sample collection and storage, in order to have a successful biomarkers analysis. Moreover, it has been highlighted how MALTI TOF MS analysis of specific proteins could suggest the selection of the best pool of samples for a powerful biomarker study. We retain that DASQ needs to be used to screen the samples to be enrolled in order to reduce the number of false positives in biomarker discovery. Due to the characteristics of MALDI, faster and less expensive than other techniques, this protocol could represent a first step in the qualitative screening of the CSF before biomarkers studies. New advanced techniques appear to be particularly promising for biomarker qualification and verification. Information about these CSF proteins can be used to develop multiple reaction monitoring (MRM) based analytical assays, which offersShow MoreRelatedDirect Analytical Sample Quality Assessment (DASQ) for Biomarker Investigation: Qualifying CSF Samples978 Words   |  4 Pagesunique window to search for new biomarkers and to improve early diagnosis of neurological diseases [1-3]. However, the complexities of the brain and human neurological disorders represent a severe roadblock to identify novel neurological biomarkers. A biomarker can be defined as a biochemical, pharmacological or physiological, indicator of a specific biological state or of a defined biological stage of an organism as represented in its characteristic specific sample. Such an indicator should be measurable

The Nature of Evil in Humanity in the Book, Heart of...

In Joseph Conrad’s novel Heart of Darkness, the cultural collisions illustrates the struggles Marlow experiences as he questions the nature of evil in humanity. Marlow compares his experience in Africa as the Romans did â€Å"nineteen hundred years ago,† they both were shocked when they got there. When Romans came long ago they did not expect to see anyone, and when Marlow came he expected something different. He thought that the natives were happy to have civilization and wanted to integrate into their society. But he was wrong, they were being treated as â€Å"criminals† and were not even respected. He knew that something else was going on there. The men that were there were just â€Å"lusty, red-eyed devils† looking for their fortune. They did not†¦show more content†¦This is similar to the brickmaker’s situation. He says that since he has no straw he cannot make bricks. He is showing his lazy characteristics when he does this. Conrad u ses of biblical allusions helps his argument of man’s inhumanity to man by showing us the evil that is in the root of civilization. Humanity is tainted with sin and evil and Conrad is highlighting the fact that some humans will always be evil. At the end of his journey he realized the reason that everyone who went on this expedition was trying to make their fortune and that is how Kurtz eventually died. His â€Å"appetite for more ivory had got the better† of him. No matter what the cost to himself and the people around him, he was going to be the best and get the most money. His selfishness eventually caused his death when the manager downstream would not send food because he hoarded all of the ivory all to himself. Marlow knew that Kurtz was very serious about his job when he saw â€Å"heads on the stakes† in front of his house. Which could have been a warning to other people who try to come get his ivory. But the interesting part was most of the heads are fac ed toward his house. Kurtz wanted attention and loved to have power. These heads could be there to adore him just like the natives when he was with them. Kurtz search for power and wealth left in its wake death and destruction, showing that humanities quest for wealth leads to destruction.Show MoreRelatedJoseph Conrad’s Heart of Darkness Illuminates the Tenebrous Core of Mankind 618 Words   |  2 Pagesintroduction of Joseph Conrad’s Heart of Darkness into society, critics have debated over the meaning of the ambiguous title. There are many interpretations of this title, but the general consensus is that in the heart of darkness, you cannot do good: you can only be less evil. The title refers to not only the physical lack of light in the jungle, but also to the grim consequences of imperialism due to the stygian heart of mankind. The first meaning is that the â€Å"heart of darkness† refers to actualRead More Light and Dark in Heart of Darkness Essay1180 Words   |  5 PagesLight and Dark in Heart of Darkness       The brightest of lights can obscure vision while darkness can contain truths: one must not be distracted by the sheen of light, which conceals the deeper reality present in darkness. Joseph Conrads novel Heart of Darkness illustrates this idea with the use of several symbols. White Europeans are used as symbols of self-deception, and objects with an alabaster quality are symbols of barriers to inner truth. Black is the foil of white; it representsRead MoreThe Journey In â€Å"Heart Of Darkness† Spans Not Only The Capricious1222 Words   |  5 PagesThe journey in â€Å"Heart of Darkness† spans not only the capricious waters extending our physical world, but also the perplexing ocean which exists in the heart of man. Through Marlow s somewhat overenthusiastic eyes, we perceive the mystery that is humanity, and the blurred line between darkness and light. It is an expedition into the deepest crevices of the human heart and mind bringing on an awareness, and fi nally descending into the abyss of hell abiding in each of us. Conrad’s use of wordplayRead MoreThe Meaning Of Heart Of Darkness Essay1138 Words   |  5 PagesNishi Natalia AP Literature Comp The Meaning of Heart of Darkness Although, as a society, we discourage the process of not judging a book by its cover, we have all been guilty of doing it at some point. The first item we look at when we pick up a new book is the title and the cover as a whole. These are two key components when it comes to using our heuristics to decide if a book is worthwhile reading or not. With only two items to judge by, each has to hold significant importance in orderRead More Achebes Misinterpretation of Conrads Heart of Darkness Essay718 Words   |  3 Pages Achebes Misinterpretation of Heart of Darkness nbsp; Joseph Conrads Heart of Darkness is heralded by many as a classic, but over the years has presented many problems of interpretation. One of the most notable misinterpretations is Chinua Achebes An Image of Africa: Racism in Conrads Heart of Darkness. In it, Achebe points to various passages in the book that supposedly prove that Conrad and his book are racist, and that the book should be cast out of the canon of classic literatureRead MoreChinua Achebe s Things Fall Apart1388 Words   |  6 PagesAfrican author criticizes the work of an old English writer who was known by many as one of the greatest novelists to write in the English language? Chinua Achebe, the author of the great African novel,  ¨Things Fall Apart ¨ argues that Joseph Conrad s book  ¨Heart Of Darkness ¨ was a false depiction of Africa and the writing was too racist and made the white race seem superior to the native Africans. The novel â€Å"Things Fall Apart† by Chinua Achebe is about the Ibo people and how they have to face the threatRead More The Light and Dark Forces in Joseph Conrads Heart of Darkness1667 Words   |  7 PagesThe Light and Dark Forces in Heart of Darkness      Ã‚  Ã‚  Ã‚   Heart of Darkness, by Joseph Conrad, explores something truer and more fundamental than a mere personal narrative. It is a night journey into the unconscious and a confrontation within the self. Certain circumstances of Marlows voyage, when looked at in these terms, have new importance. Marlow insists on the dreamlike quality of his narrative. It seems to me I am trying to tell you a dream - making a vain attempt, because no relation ofRead MoreHow Does Conrad Link His Physical Exploration to a Psychological Journey of Discovery?1412 Words   |  6 PagesThe â€Å"Heart of Darkness† is a tale of passage and discovery, not only into the heart of Africa, but into the heart of our human mind. Written by Joseph Conrad, this novel follows Marlow’s expedition into the unknown depths of the Congo in search of Kurtz and his adored wisdom. Conrad links Marlow’s physical journey to a psychological quest of discovery into evil and darkness inside each one of us. Through the impassable landscape, the language barrier between the colonists and the natives, and embodimentRead MoreEssay on Modernism in Literature1532 Words   |  7 Pagesprocesses led to the belief of superior races and people groups, which in part, caused World War I. People became cynical, people became evil, and people became destructive. Through the work of writers such as T.S. Eliot, W.B. Yeats, and Joseph Conrad, modernism in literature shed light on the true nature of the heart of humanity. With this newly incorporated view of humanity, a new theory emerged involving psychology and the behaviors of men and women. Sigmund Freud was an Austrian neurologist who foundedRead More Ambiguities Explored in Joseph Conrads Heart of Darkness Essay1458 Words   |  6 PagesAmbiguities Explored in Heart of Darkness   Ã‚  Ã‚   Literature is never interpreted in exactly the same way by two different readers. A prime example of a work of literature that is very ambiguous is Joseph Conrads, Heart of Darkness. The Ambiguities that exist in this book are Marlows relationship to colonialism, Marlows changing feelings toward Kurtz, and Marlows lie to the Intended at the end of the story.    One interpretation of Marlows relationship to colonialism is that

Commentary on Adrian Henris Tonight at Noon Essay Example For Students

Commentary on Adrian Henris Tonight at Noon Essay Tonight at noon is a poem that contradicts itself from the title right up to the ending using much of general knowledge and just meandering the reality around, to create absurdity in the poem. Henri has used paradox throughout the whole poem swapping everything he mentions around. He starts using this practice since the title, Tonight at Noon as it is impossible because noon cant ever be at night as it is during midday. The words tonight at noon can be seen at least once per stanza, which shows repetition throughout the poetry. The repetition of the title is effective as Henri keeps on showing that he is constantly contradicting himself. This poem actually has no set arrangement to it, and it seems to run like a river, all soft and smooth. There is no real structure for it except for the fact that it continues on from each other. The idiom used in this poem is effortless to comprehend. There is not complex diction used in this poem, which shows that it has been targeted to a wider assembly of audience, most probable to be from the middle class. The ideas used are very simple and easy to attach yourself to which reaches Henris goal so that a wider audience has an thoughtful understanding of his poetry. In the last stanza, there is an uncomfortable image being presented when he talks about graveyards with the dead burying people alive. There is also an image of sadness shown on his second last line of the poem, when he says you will tell me you love me. This line is considered as a line with sadness hidden behind it as he is trying to make someone tell him that they love him, as if he is lacking love so he actually has to ask for love himself as no one is essentially enthusiastic to give him their love out of their own free will. Children who are unhappy with their own families are sent to live in homes, and not children from happy families as Henri states in the first stanza. The impossibility of this fact makes this line humorous as everyone knows that this will never happen in real life. Elephants cant tell jokes, whereas humans always tell jokes about elephants. This line is comical as it shows how extreme Henris thoughts are. How can America declare peace on Russia if they never even started a war? This is another example of Henrys comical thoughts. The first daffodils come in spring, and not autumn when the leaves actually fall off trees, downwards. Pigs flying are another of his mad thoughts as you can only find that in fantasy stories. There is no black house for equal rights demonstrations. And white Americans will not even bother about those demonstrations. Here, Henri is just swapping two words around, for low class humour for anyone who doesnt know about a certain white house in America. It is normal to see girls in bikinis bathing under the sun, as they get a tan, but what is the point of moon bathing except if you are planning on stargazing due to the fact that you cant ever possibly get a tan from tanning without sunlight or some sort of UV rays. Art galleries are frequented by an older audience, so if they are closed to people over the age of twenty one, how can these galleries have any source of income at all, considering barely anyone under the age of twenty one actually goes into one of those shows. The purpose of the top twenty is not for poetry, but for hip music, for example pop, rock, and rnb. .ufb5038f10595e9a738e2524943c67603 , .ufb5038f10595e9a738e2524943c67603 .postImageUrl , .ufb5038f10595e9a738e2524943c67603 .centered-text-area { min-height: 80px; position: relative; } .ufb5038f10595e9a738e2524943c67603 , .ufb5038f10595e9a738e2524943c67603:hover , .ufb5038f10595e9a738e2524943c67603:visited , .ufb5038f10595e9a738e2524943c67603:active { border:0!important; } .ufb5038f10595e9a738e2524943c67603 .clearfix:after { content: ""; display: table; clear: both; } .ufb5038f10595e9a738e2524943c67603 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .ufb5038f10595e9a738e2524943c67603:active , .ufb5038f10595e9a738e2524943c67603:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .ufb5038f10595e9a738e2524943c67603 .centered-text-area { width: 100%; position: relative ; } .ufb5038f10595e9a738e2524943c67603 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .ufb5038f10595e9a738e2524943c67603 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .ufb5038f10595e9a738e2524943c67603 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .ufb5038f10595e9a738e2524943c67603:hover .ctaButton { background-color: #34495E!important; } .ufb5038f10595e9a738e2524943c67603 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .ufb5038f10595e9a738e2524943c67603 .ufb5038f10595e9a738e2524943c67603-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .ufb5038f10595e9a738e2524943c67603:after { content: ""; display: block; clear: both; } READ: Background to Poem EssayThe fact that Henri mentions having poetry in the top twenty is as if he is comparing contemporary poetry to twenty first century music which isnt really comparable as their differences are so widespread. Back alleys are small narrow roads which are dark, and provide teenage lovers a private space to fool around, normally have no sunlight passing through it whatsoever. To conclude, this poem is very comical and serves as a way to get people to laugh, and I find that Henri has attained this very successfully as this poem can make anyone laugh at anytime due to his constant contradicting facts throughout the whole poem.